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Figure 4 | BMC Biochemistry

Figure 4

From: Mammalian target of Rapamycin inhibition and mycobacterial survival are uncoupled in murine macrophages

Figure 4

Rapamycin does not kill M. smegmatis or BCG in the absence of macrophages. M. smegmatis (A) or BCG (B) were cultured in DMEM + DMSO (control) or the indicated doses of Rapamycin. After 3 hours at 37C and 5% CO2, the wells were mixed and the bacteria were plated for CFU determination. On the left are the raw CFU values for each replicate. On the right is the percentage change (+/− SEM) from the mean value of cells treated with DMSO, which was set at 100%. For both (A) and (B), shown are the combined results of two independent assays.

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