Figure 10

Assessing the influence of 5′ leader sequences on BNIP-2 precursor patterns. The contribution of alternative translation initiation to the multiple precursor bands was assessed by transfecting HeLa and NIH/3T3 cells with various uTIS mutated BNIP-2 expressing constructs. Whereas mutation of uTIS A did not alter the precursor pattern observed in the human and mouse BNIP-2 control setups, mutation of uTIS B (black arrow) resulted in the disappearance of the precursor band with the highest MW in murine BNIP-2 variants. The dashed arrow is indicative for the N-terminally extended BNIP-2 variant raised upon translation initiation at uTIS C. The BNIP-2 Swiss-Prot database annotated precursor band is annotated with a grey arrow (cfr. Additional file 7: Figure S5).