Figure 6
From: Chemical-genetic induction of Malonyl-CoA decarboxylase in skeletal muscle
MCD induction exacerbates defects in diet-induced insulin signaling. Western blot of gastrocnemius muscle isolated from Tg-fMCDskel and control mice on LFD or HFD given Shield-1 (60 mg/kg) or vehicle for 5 days. Mice were fasted for 6 hours before a 10 min insulin stimulation. (A) AKT Ser 473 phosphorylation, normalized to total protein and quantified. (B) IRS-1 Ser 302 phosphorylation, normalized to total IRS-1 and quantified. (C) GSK3β Ser 9 phosphorylation, normalized to total GSK3β and quantified. (D) Glycogen Synthase (GS) Ser 641 phosphorylation, normalized to total Glycogen Synthase and quantified. Data are expressed as means +/- SEM. *p < 0.05 by one-way ANOVA.