Figure 4

Normalized equilibrium curves of apoform (A) and holoform (B) of HmuY protein variants. Protein samples at concentration 40 μM in 20 mM sodium phosphate buffer, pH 7.4, containing 20 mM NaCl and Sypro Orange were heated from 25°C to 80°C with a rate of 0.5°C per 30 s. Probe fluorescence was monitored with excitation at 585 nm and emission at 610 nm. Representative data out of three independent experiments performed in triplicate are shown.