Figure 3

Normalized equilibrium curves of apoform (A) and holoform (B) of HmuY protein variants. Denaturation was carried out using 4 μM protein samples in 20 mM sodium phosphate buffer, pH 6.5, containing 20 mM NaCl and 1 M GdnHCl. Samples ware heated in 10-mm path-length cells with a rate of 1°C per min. Spectra ware monitored with excitation at 295 nm and emission at 315 nm. Representative data out of three independent experiments performed in triplicate are shown.