Figure 1

Transformed IMR90 cells show characteristics of a cancer cell. (A) Western blot confirming the expression of the three genetic factors Ras, hTERT and SV 40 Large T in the transformed IMR90 primary human cells. The expression of hTERT on the western blot was detected using anti-FLAG antibody. (B) Changes in cellular morphology after RSH transformation. Transformation of IMR90 cells and resulted in shorter and rounder cells. Left bottom corners show the enlarged pictures. (C) Soft agar assay determining the anchorage independence of the transformed RSH cells in vitro. MCF-7 cells were used as the positive control. The experiment was carried out in triplicates. Representative image of one well is shown. (D) Survival of IMR90 control and IMR90 RSH cells in serum-free medium. (E) Cells of IMR90 control and IMR90 RSH were treated with 1 μM, 3 μM and 5 μM of doxorubicin separately for up to 48 hours. (F) G-band Karyotype analysis of IMR90 control cells infected with control vector and IMR90 RSH cells. Arrows indicate the presence of genetic aberrations. (G) Metaphase spreads of IMR90 RSH cells. White arrows indicate the presence of genetic aberrations. (H) A representative spectral karyotype of a metaphase from IMR90 RSH cells. Recurrent abnormality is defined as at least 3 metaphase cells having the abnormality at the same region of chromosomal location. Chromosomal abnormality can be observed at chromosomes 4, 18, 20.