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Table 1 Yield and purification fold at different steps of purification of urease from germinated pisum sativum seeds

From: Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds

Step of purification Total protein (mg/ml) Total activity (Units) Specific activity (U/mg) Yield (%) Purification fold
Crude extract 22 10000 454.5 100 1
Acetone precipitation and dialysis 12 7500 625 75 1.3
Ion exchange DEAE-cellulose 3.1 5000 1613 50 3.5
Gel filtration Sephacryl S-200 0.72 4200 5833 42 12.85