Table 1 Yield and purification fold at different steps of purification of urease from germinated pisum sativum seeds
Step of purification | Total protein (mg/ml) | Total activity (Units) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
Crude extract | 22 | 10000 | 454.5 | 100 | 1 |
Acetone precipitation and dialysis | 12 | 7500 | 625 | 75 | 1.3 |
Ion exchange DEAE-cellulose | 3.1 | 5000 | 1613 | 50 | 3.5 |
Gel filtration Sephacryl S-200 | 0.72 | 4200 | 5833 | 42 | 12.85 |