Skip to main content
Figure 5 | BMC Biochemistry

Figure 5

From: Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs

Figure 5

Secretion of dNGLUC is induced by growth factor stimulation. A, 293 T cells were transfected with dNGLUC and cultured overnight. Cells were washed once in PBS and fresh media was added. Supernatants were collected at the indicated time points prior to analysis on a PerkinElmer Envision multilabel plate reader. B, C, 293 T cells expressing dNGLUC were cultured for 24 h and then starved in 0.1% FCS overnight. Media containing serum (full line) or no serum (dotted line) was added back to the cells and luciferase activity in supernatants was measured after 4 h (B) or at the indicated time points (C) using a PerkinElmer Envision II plate reader. Shown is the mean of 5 independent transfections with standard deviation. D, COS-7 cells were transiently transfected with pEAK12-dNGLUC using calcium phosphate precipitation, cultured for 24 h and then starved overnight in 0.1% FCS. Cells were then stimulated with 100 ng/mL EGF at 37°C for 2 h and supernatants were harvested. C-D, shown is the mean of three experiments plus standard deviations. R.L.U. – relative light units, SN - supernatant.

Back to article page