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Figure 6 | BMC Biochemistry

Figure 6

From: The basic amino acids in the coiled-coil domain of CIN85 regulate its interaction with c-Cbl and phosphatidic acid during epidermal growth factor receptor (EGFR) endocytosis

Figure 6

Cellular colocalization of Vps4 and CHMP2A with CIN85 domains. Τhe bar is 10 μm. (A) Construction of fusion proteins of CIN85 fragment. CIN85 fragment was tagged with eGFP. (B) Intracellular localization of Vps4 and Vps4m (ATPase negative mutant). The proteins were tagged with DsRed and expressed in HEK293 cells. (C) Colocalization of CIN85 fragment with CHMP2A. EGFP-tagged CIN85 mutant and DsRed-tagged CHMP2A were co-expressed in HEK293 cells. The proteins were visualized by the tagged fluorescence protein. (D) Colocalization of CIN85 fragment with Vps4m.

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BMC Biochemistry

ISSN: 1471-2091

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