Figure 2

Amplification of the TVAG_254130 open reading frame from T. vaginalis cDNA. Total RNA was isolated from a 40 hr culture of T. vaginalis grown under our standard conditions. The resulting RNA was used to synthesize cDNA (+RT). To control for genomic DNA contamination, mock reactions were carried out in which reverse transcriptase was omitted from the cDNA synthesis step (-RT).