Figure 1
Sucrose is unable to support the growth of T. vaginalis . TY medium supplemented with maltose (0.5% by weight; TYM), sucrose (0.5% by weight; TYS), or that had no added sugar (TY) was prepared. T. vaginalis was grown for 40 hr in TYM. The cells were collected, washed with TY medium and then used to inoculate this same medium, TYM, or TYS at an initial density of 1 x 105 cells/ml. The cell density was then recorded after 16, 24, 40 and 48 hr of growth. Only TYM medium supported robust growth of T. vaginalis, with a decrease in motile organisms noted by 48 hr in both TY and TYS media. The results shown are the mean ± standard error for four independent determinations.