Figure 3

Western immunoblot analysis. (A) Proteins extracted from Canton S third instar larval whole bodies. The blots were probed with a Guinea pig polyclonal anti-OSCP1 antibody. In the immunoblotting, both 35 kDa and 70 kDa bands were detected. The 35 kDa band corresponds to dOSCP1 protein. The faint 70 kDa band likely represents the dimeric form. (B) Protein extracts of eye imaginal discs from GMR-Gal4; +; + (control flies) and GMR-Gal4; UAS-dOSCP1IR; + (knockdown flies). Knockdown of dOSCP1 led to decrease in the 35 kDa band when compared with control flies. (C) Quantification of the 35 kDa band using Image Saver 6 software. The relative expression level was significantly decreased in knockdown flies (*P < 0.001; Student t test; n = 3; 30 μg of proteins per replicate). α-Tubulin was used as a loading control.