Figure 2

Bacterial lysate activity assay. A) Hydrogen evolution activities of hydrogenases bearing select substitutions as ascertained by by bacterial lysate assay. Error bars represent the standard error of the mean activity. The dotted line separates Asp substitution data from Asn substitution data. B) SYPRO Ruby stain loading control for C) anti-HynL and D) anti-Strep western blots of bacterial lysates of select substitutions demonstrating approximate enzyme yield.