Table 4 Apparent affinities of clones from D5-Lib-II selection against 5-Helix

From: Side chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the V_H1-69 germline segment

	On-phage (bivalent)		Off-phage (monovalent)
Clone	IC₅₀for 5-Helix^A/nM	IC₅₀for 6-Helix-Fd^A/nM	IC₅₀for 5-Helix^A/nM	IC₅₀for 6-Helix-Fd^A/nM
D5 WT	0.1 (0.07-0.1)	11 (8–15)	7.3 (5.3-10)	290
25B8	3.1 (1.8-5.3)	~100	ND^B	ND^B
25C10	1.8 (1.3-2.7)	28 (21–37)	56 (40–79)	NC ^C
25D8	5.8 (4.2-7.5)	~3000	ND^B	ND^B
25A10	1.6 (1.3-2.0)	260 (150–450)	5.4 (3.8-7.7)	~500
25D9	1.7 (1.4-2.1)	230 (150–360)	ND^B	ND^B
25G8	7.2 (4.2-12.3)	300 (120–750)	ND^B	ND^B
25C6	32 (24–44)	~1000	ND^B	ND^B
25C12	100 (31–300)	~1000	ND^B	ND^B
25D6	0.5 (0.3-0.8)	~80	ND^B	ND^B
25D3	17 (9.9-30)	170 (70–390)	ND^B	ND^B
25F1	0.9 (0.7-1.2)	40 (20–79)	ND^B	ND^B
25B6	0.3 (0.2-0.4)	23 (15–36)	0.6 (0.3-1.0)	94 (56–160)
25F10	0.2 (0.2-0.3)	22 (16–30)	ND^B	ND^B
25A12	2.2 (1.2-4.0)	220 (70–670)	34 (23–52)	280 (120–640)

^A 95% Confidence intervals from data fitting are shown in parentheses.
^B ND, not determined.
^CNC, no convergence observed upon data fitting, suggesting weak or insignificant binding.

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