Table 2 Library design
D5 position | Contact scoreA | Amino acid distribution (frequency)B | Designed codonC | Encoded residues (coverageD) |
|---|---|---|---|---|
LCDR1 | Â | |||
E27 | Low | E(3)/Q(13)/S(2) | Not varied | E (17%) |
G28 | Mid | D*(5)/G(2)/N(1)/Q(1)/S(8)/Y*(1) | RRC | D/N/G/S (88%) |
Y30 | Mid | G(3)/R(1)/S*(10)/Y*(2)/V(1) | NDT | C/D/F/G/H/I/N/L/R/S/V/Y (100%) |
H31 | Mid | H(4)/N*(5)/S(7)/T(2) | NDT | C/D/F/G/H/I/N/L/R/S/V/Y (61%) |
W32 | High | A(2)/D(1)/F(1)/G(1)/M(1)/N(2)/S(1)/W*(2)/Y*(6)/- (1) | NDT | C/D/F/G/H/I/N/L/R/S/V/Y (56%) |
LCDR2 | Â | |||
Y49 | High | Y*(18) | TMT | S/Y (100%) |
K50 | High | A(2)/D(1)/G(5)/K*(3)/L(2)/S(2)/Y*(3) | HRC | C/H/N/R/S/Y (28%) |
S52 | Low | S(17)/T(1) | TMT | S/Y (94%) |
S53 | Mid | F*(2)/N(3)/R*(3)/S(6)/T(3)/Y(1) | HRC | C/H/N/R/S/Y (73%) |
A55 | Low | A(9)/F(1)/H(3)/K(1)/P(1)/Q(1)/Y(2) | Not varied | A (50%) |
LCDR3 | Â | |||
Y91 | High | F(1)/G(3)/H(2)/R*(1)/S(3)/W*(2)/Y*(6) | HRC | C/H/N/R/S/Y (67%) |
S92 | High | A(1)/D*(1)/G(4)/L(1)/N*(3)/S*(4)/T(1)/W(1)/Y(2) | KMT | A/D/S/Y (44%) |
N93 | High | A(1)/D(1)/G(4)/H(1)/N*(5)/Q(1)/S*(1)/T(4) | RVC | A/D/G/N/S/T (89%) |
Y94 | Mid | L(3)/N(2)/S*(5)/T(3)/V(1)/W(2)/Y*(1)/- (1) | DMT | A/D/N/S/T/Y (61%) |
P95 | Mid | L(2)/P*(16) | Not varied | P (89%) |
L96 | High | F*(2)/I(1)/L*(1)/P(5)/R(1)/S(1)/W(4)/Y(3) | YDT | C/F/H/L/R/Y (39%) |
T97 | Low | R(1)/T(17) | Not varied | T (94%) |