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Table 1 Library design

From: Side chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the VH1-69 germline segment

 

Amino acid substitutionsA

  

Library

LCDR1B (D5 WT permitted)

LCDR2

LCDR3

HCDR3B (D5 WT permitted)

Theoretical diversity

Actual diversity

D5-Lib-I

A/D/H/P/S/Y

A/D/H/P/S/Y

A/D/H/P/S/Y

A/D/H/P/S/Y

8 x 1017

3 x 109

D5-Lib-II

TailoredC

TailoredC

TailoredC

A/C/D/E/G/K/N/R/S/T/Y/W

8 x 1019

3 x 109

  1. A Amino substitutions encoded by limited diversity codon sets. For LCDRs 1 – 3: BMT (D5-Lib-I), or tailored codon sets listed in Table 2 (D5-Lib-II); for HCDR3 in all libraries: DVK. Nucleotide degeneracies: M = A/C, B = C/G/T, D = A/G/T, V = A/C/G, K = G/T.
  2. B In LCDR1 and HCDR3, the library synthesis was performed in such a manner to allow the D5 WT sequence as an alternative to the library sequences.
  3. C Tailored diversity codon sets used as shown in Table 2.
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BMC Biochemistry

ISSN: 1471-2091

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