Figure 10From: Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtiiExpressions of CrCIS gene in E. coli BL21 and enzyme activity detected in vitro. After induced by IPTG and cultivated in 0, 2, 4, and 6 hours, total protein had been harvested and run through SDS-PAGE. A) Expression of CrCIS in E. coli BL21; B) Purified GST-CrCIS; C) Enzyme activity of GST-CrCIS detected in vitro.Back to article page