Figure 3
Effect of GTPγS on the fluorescence anisotropy of F 2 FlAsH-G-protein preparations. The fluorescence anisotropy of α-subunits with (A, B) or without (C, D) βγM subunits, in the absence (solid bars) or presence of 10 μM GTPγS (striped bars), was measured after 6 h of incubation at 28°C (A and C) and again after subsequent heat treatment for 1 h at 70°C, (B and D). Data is from two independent experiments carried out in duplicate, presented as mean ± SD as error bars.