Figure 1
The effect of 10 μM GTPγS on the formation of complexes between F 2 FlAsH and G-proteins in the presence (A) or absence of βγM subunits (B). Data are presented as the time-dependent attenuation of fluorescence anisotropy in comparison to GTPγS-untreated F2FlAsH-G-protein complexes. The preparations used were αolf (1), αq (2), αsshort (3), αslong (4), α13 (5), none (6) and βγM (7). Data is from two or three independent experiments carried out in duplicate and presented as mean ± SD as error bars.