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Table 4 Steady state kinetic parameters of the wild type and mutant proteins a

From: The mononuclear metal center of type-I dihydroorotase from aquifex aeolicus

Protein

Km

Vmax

 

mM

μmol/min

Wild Type

1.52 ± 0.30

0.250 ± 0.017

Wild Type + 5-fold ZnCl2

1.93 ± 0.35

0.270 ± 0.018

Wild Type

1.03 ± 0.16

0.233 ± 0.011

His180Ala

1.65 ± 0.26

0.238 ± 0.013

His232Ala

1.34 ± 0.22

0.224 ± 0.012

His180Ala/His232Ala

1.55 ± 0.30

0.186 ± 0.012

  1. aThe dihydroorotate saturation curves (Figures 4 and 7) were fit by a least squares analysis to the Michaelis Menten equation using the program KaleidaGraph.
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BMC Biochemistry

ISSN: 1471-2091

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