Table 3 Zinc analysis a of wild type and mutant proteins
From: The mononuclear metal center of type-I dihydroorotase from aquifex aeolicus
Protein | EDTA | Zn | Zn/DHO | |
|---|---|---|---|---|
|  | μM | molar ratio | 25°C | 75°C |
ATC | 0 | 0 | 0 | 0 |
Wild Type DHO | ||||
DHO | 0 | 0 | 0.84 | 0.83 |
DHO | 0 | 5X | 1.07 | 0.97 |
DHO | 100 | 5X | 0.98 | 1.01 |
DHO-ATC | 0 | 0 | 1.06 | nd |
DHO-ATC | 0 | 5X | 0.98 | 0.95 |
DHO-ATC | 100 | 5X | 0.95 | 0.98 |
DHO Double Mutant | ||||
DHO | 0 | 0 | 0.71 | 0.90 |
DHO | 0 | 5X | 0.94 | 1.27 |
DHO | 100 | 5X | 0.98 | 1.30 |
DHO-ATC | 0 | 0 | 1.20 | nd |
DHO-ATC | 0 | 5X | 0.93 | 1.14 |
DHO-ATC | 100 | 5X | 0.95 | 1.29 |