Figure 7From: The mononuclear metal center of type-I dihydroorotase from aquifex aeolicusSteady state kinetics of the wild type and β-site mutants. A dihydroorotate saturation curve of DHO-ATC assayed as described in the Methods (250 mM Tris acetate, pH 8.3 at 72°C) was determined for the wild type (●), His180Ala (○), His232Ala (■) and the His180Ala/His232 (□). The kinetic parameters are summarized in Table 4.Back to article page