Biosynthesis and release of pheromonal bile salts in mature male sea lamprey

BMC Biochemistry

Table 2 Real-time quantitative PCR sequence information

Gene ID	NCBI accession no.	Sequence for real-time quantitative PCR
cyp7a1	PMZ0014378-RA	CAACATGTCGGCGCTCATC GCCCTCCGAATACAACTCA ATGACACGCTGTCTCGCATG
cyp27a1	PMZ0003691-RA	TCTGGCCAAAATGTCATTCCT TAAGGCTGTCATCAAAG AGATTCTCAGACTGTATCCAGTGGTGCC
cyp8b1	PMZ0014367-RA	TCCCCTTGATAAAGGCCTCAT CCCATGGCTGGGCCACGCCATCGAGTTCCGGAGGGA CATGTACGCCTTCCTGCGG
bsep	PMZ0007614-RA	GTGTCTCAGGAGCCGGTGTT GTTCGACTGCAGCATTG CCGACAACATTCGCTACGGTGCC
slc10a1	PMZ0014657-RA	CTGTCCCGGAGGGAACCT CTCCAACGTGTTCGCGCTGGCGCTCGACGGAGACATGAA CCTCAGCATCCTCATGACCACGTG
slc10a2	PMZ0006503-RA	GCTGGCGCTGGTGATGTT CGCCATGGGCTGCACGGTGCAGATTCACAA GGTGATTGCTCACCTTCGCAATCC
hsd3b7	PMZ0008058-RA	CGGCCGTTCTACAAGTTCGT CCCACCCATCAACCGTCAGCTCGT GGTCATGGTCAACACGCACTTCA
sult2b1	PMZ0018743-RA	GGAACGTTCATGCGAAAAGG CGCAGTTGGCAACTGGAAAAG TGAATTCACCGTGGCGTTGA
sult2a1	PMZ0001223-RA	TGGTCACCTACCCCAAATCAG GCACGACGTGGATGCAGGAGA TCGTGACCCTGGTGTATAGCGACGGGGACCTG
sult1c1	PMZ0016497-RA	CCGTGATGCGCGACAAC CCCATGACGAATTACAGCACCTTGCC CACCGACTTCTTGGACCACTCCGTG
40s	PMZ0000703-RA	ACCTACGCAGGAACAGCTATGAC CATCTCGAGCAGCTGAA GCTCCAATGTGGTGGAATTCGTCG

Partial sequences selected for amplification in RTQ-PCR experiments are shown, along with the NCBI accession number for each gene. Italicized sequences indicate the 5 and 3 primers for each gene. Sequences used for TaqMan MGB probes are shown in bold font. Note that the 3′ primer is the reverse compliment of the sequence shown above.

ISSN: 1471-2091