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Figure 3 | BMC Biochemistry

Figure 3

From: Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis

Figure 3

Constructs of the different YedY enzymes expressed in this study. The enclosed numbers refer to the proteins studied in this work: (1) corresponds to the C-ter tagged protein (31.4 kDa); (2) is the N-ter tagged enzyme lacking the signal sequence (-SS; 32.6 kDa); (3) is the N-ter tagged protein with the signal sequence (+SS; 32.6 kDa); and (4) is the untagged protein (30.4 kDa). The “V” symbol indicates the position of the TEV protease recognition sequence for cleavage.

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BMC Biochemistry

ISSN: 1471-2091

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