Figure 2

Interaction between KCTD20 and Akt or a catalytic subunit of protein phosphatase in COS7 cells. A and B, GST-Akt (A) or GST-protein phosphatase catalytic subunit (B) and His-Xpress-KCTD20 were coexpressed in COS7 cells by transfection. pEF4/KCTD20 encoded N-terminally His-Xpress-tagged KCTD20. pEBG vectors encoded N-terminally GST-tagged proteins (Akt1, Akt2, Akt3, the catalytic subunit of PP1A (PP1Ac), and the catalytic subunit of PP2A (PP2Ac)). The pEBG backbone vector (named vector) encoded GST. GST-pulldown assays with glutathione sepharose beads were performed using the cell lysates. His-Xpress-KCTD20, co-precipitated with GST-Akt (A) or GST-catalytic subunit of protein phosphatase (B), was detected with anti-Xpress antibody (upper panel) and anti-GST antibody (lower panel).