|
Dairy cows
|
Total SA ± SD (n = 3) nmole/min/mg
|
CV%
|
Holo SA ± SD (n = 3) nmole/min/mg
|
CV%
|
|---|
|
1
|
15.249 ± 0.543
|
3.56
|
0.467 ± 0.035
|
7.57
|
|
2
|
12.027 ± 0.973
|
8.09
|
0.561 ± 0.036
|
6.37
|
|
3
|
15.415 ± 0.631
|
4.10
|
0.489 ± 0.012
|
2.46
|
|
4
|
13.091 ± 0.397
|
3.03
|
0.513 ± 0.046
|
8.88
|
|
5
|
14.552 ± 0.881
|
6.05
|
0.352 ± 0.002
|
4.65
|
|
6
|
12.714 ± 0.405
|
3.18
|
0.676 ± 0.014
|
2.09
|
- Assays were performed in presence of 200 μM of AdoCbl and 400 μM of MMCoA. Incubation was conducted at 37°C for 30 min. The amounts of protein used in the assays were 40 μg. Specific activities (SA) are expressed as nmoles of succinyl-CoA/min/mg protein. Standard deviation (SD) and coefficients of variation (CV%) are also presented.
