Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity

Table 5 Specific activity of MCM measured in liver of dairy cows at their peak of lactation

Dairy cows	Total SA ± SD (n = 3) nmole/min/mg	CV%	Holo SA ± SD (n = 3) nmole/min/mg	CV%
1	15.249 ± 0.543	3.56	0.467 ± 0.035	7.57
2	12.027 ± 0.973	8.09	0.561 ± 0.036	6.37
3	15.415 ± 0.631	4.10	0.489 ± 0.012	2.46
4	13.091 ± 0.397	3.03	0.513 ± 0.046	8.88
5	14.552 ± 0.881	6.05	0.352 ± 0.002	4.65
6	12.714 ± 0.405	3.18	0.676 ± 0.014	2.09

Assays were performed in presence of 200 μM of AdoCbl and 400 μM of MMCoA. Incubation was conducted at 37°C for 30 min. The amounts of protein used in the assays were 40 μg. Specific activities (SA) are expressed as nmoles of succinyl-CoA/min/mg protein. Standard deviation (SD) and coefficients of variation (CV%) are also presented.

ISSN: 1471-2091