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Table 4 Specific activity of MCM measured in liver obtained from slaughterhouse

From: Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity

Runs

Total SA ± SD (n = 5) nmole/min/mg protein

CV% intra-run (n = 5)

CV% inter-run (n = 45)

Run 1

5.563 ± 0.200

3.60

 

Run 2

6.569 ± 0.333

5.07

 

Run 3

6.015 ± 0.281

4.68

 

Run 4

6.465 ± 0.378

5.85

 

Run 5

6.234 ± 0.502

8.05

 

Run 6

5.558 ± 0.166

2.98

 

Run 7

6.150 ± 0.106

1.72

 

Run 8

5.677 ± 0.051

0.90

 

Run 9

5.317 ± 0.173

3.25

 

Mean SA

5.950 ± 0.44

 

7.40

  1. Assays were performed in presence of 200 μM of AdoCbl and 400 μM of MMCoA. Incubation was conducted at 37°C for 30 min. The amounts of protein used in the assays were 66 μg. Specific activities (SA) are expressed as nmoles of succinyl-CoA/min/mg protein. Standard deviation (SD) and coefficients of variation (CV%) are also presented.