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Table 3 Stability of succinyl-CoA after completion of experiences and quality control test with succinyl-CoA standard solutions

From: Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity

Post-process stability

Sample ID

Injection count

Time after the first injection (hours)

Mean concentration of SSCoA (μM)

CV% inter-injection

Bovine-liver-1

2

33

33.74

9.22

Bovine-liver-2

2

39

65.59

7.25

Bovine-liver-3

2

40

56.11

6.67

Bovine-liver-4

2

45

51.20

4.55

Standard (SSCoA)

 

High Qc

Middle Qc

Low Qc-2

Low Qc-1

Nominal value

 

500 μM

62.5 μM

7.8 μM

3.9 μM

day 1

     
  

485.14

59.99

7.11

3.53

  

415.51

63.46

7.12

3.98

  

414.23

51.80

10.20

4.29

 

Mean (n = 3)

438.29

58.42

8.15

3.93

 

CV%

9.26

10.25

21.87

9.67

day 2

     
  

441.52

67.30

7.86

3.75

  

435.70

56.49

7.97

3.72

  

433.57

56.71

7.86

3.51

 

Mean (n = 3)

436.93

60.16

7.90

3.66

 

CV%

0.94

10.27

0.83

3.61

day 3

     
  

401.95

64.41

8.39

3.52

  

405.45

64.14

8.48

3.67

  

403.39

62.29

8.53

3.48

 

Mean (n = 3)

403.60

63.61

8.47

3.56

 

CV%

0.44

1.82

0.86

2.79

CV% inter-days (n = 9)

6.23

8.08

11.33

7.20

Accuracy%

85.25

97.17

104.75

95.29

  1. The post-process stability study used 66 μg of protein over 30 min of incubation at 37°C in presence of 200 μM of AdoCbl and 400 μM of MMCoA. The quality control (Qc) study used four concentrations of the standard succinyl-CoA. The calculated accuracies varied from 85% to 104%.
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BMC Biochemistry

ISSN: 1471-2091

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