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Table 2 Recovery and stability of succinyl-CoA according to sample storage condition

From: Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity

Recovery data

Level of protein tested (μg)

Level of standard added (μM)

n

Recovery%

CV%

33

75

4

73.79

12.61

66

75

6

83.97

6.46

166

75

3

70.83

14.72

Sample storage stability

 

n

Mean Conc (μM)

CV%

CV% inter-day

% difference

Fresh liver

     

day 1

5

60.88

1.72

 

day 1 vs day 2: 7.7

day 2

5

56.20

0.90

 

day 2 vs day 3: 6.3

day 3

5

52.64

3.25

 

day 1 vs day 3: 13.5

 

15

  

7.30

 

Nitrogen snapped Liver

     

day 1

5

58.72

5.05

 

day 1 vs day 2: 11.6

day 2

5

51.93

8.21

 

day 2 vs day 3: 5.8

day 3

5

48.94

1.39

 

day 1 vs day 3: 16.7

 

15

  

9.43

 

Fresh vs Nitrogen

30

54.89

 

8.21

6

Control standard 75 μM

    

Accuracy%

day 1

2

60.84

17.99

  

day 2

2

69.80

10.82

  

day 3

2

68.70

1.06

  

day 4

2

64.69

0.51

  

day 5

2

65.83

3.23

  

day 6

2

69.57

6.84

  
 

12

66.57

 

8.18

88.76

  1. The best recovery ratio was observed with 66 μg of protein. The results of storage stability correspond to the mean concentration of SSCoA (μM) formed by 66 μg of protein over 30 min of incubation at 37°C in presence of 200 μM of AdoCbl and 400 μM of MMCoA. Two vials of a known concentration of the standard (75 μM) were included as quality control.
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BMC Biochemistry

ISSN: 1471-2091

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