Table 1 Limits of quantification of succinyl-CoA and methylmalonyl-CoA

Nominal concentration (μM)

Mean calculated concentration (μM)

CV%n = 4

Accuracy%

7.81

6.74

10.38

86.33

15.62

15.35

1.22

98.28

31.25

30.72

1.20

98.32

62.5

63.98

1.65

102.37

125

125.71

0.40

100.57

250

249.47

0.15

99.79

500

497.58

0.34

99.52

Methylmalonyl-CoA

Nominal concentration (μM)

Mean calculated concentration (μM)

CV%

Accuracy%

n = 4

15.62

15.33

1.34

98.12

31.25

31.20

0.10

99.85

62.5

63.65

1.28

101.83

125

125.55

0.31

100.44

250

249.47

0.15

99.79

500

495.82

0.59

99.16