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Figure 3 | BMC Biochemistry

Figure 3

From: Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity

Figure 3

Velocity of MCM versus increasing concentration of substrate after 30 min of incubation at 37°C. The results are based on the mean concentration of succinyl-CoA (SCCoA) for 2 assays; we tested 7 substrate (methylmalonyl-CoA, MMCoA) concentrations (4.69-300 μM) using 66 μg of protein and 200 μM of AdoCbl. Each concentration level was duplicated in each assay. Error bars are the Standard Error (SE).

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BMC Biochemistry

ISSN: 1471-2091

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