Figure 2

Formation of succinyl-CoA according to protein amount or time of incubation. A) Rate of formation of succinyl-CoA per min according to the amount of protein used in the assay after 30 min of incubation at 37°C. Each amount of protein was tested 4 times and the initial concentrations of AdoCbl and MMCoA were 200 μM and 400 μM respectively. B) Concentration of succinyl-CoA (μM) formed by 66 μg of protein at 37°C as a function of incubation time (expressed in minutes). The initial concentration of AdoCbl and MMCoA were 200 μM and 263 μM respectively. Tests were done twice at 5, 10 and 40 minutes, five times at 0 minute and 8 times at 15, 20, 25 and 30 minutes. Values are presented as the mean ± SE (Standard Error).