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Figure 5 | BMC Biochemistry

Figure 5

From: Identification of the lamin A/C phosphoepitope recognized by the antibody P-STM in mitotic HeLa S3 cells

Figure 5

SILAC-based quantification of tryptic peptides of lamins A and C immunoprecipitated from HeLa S3 cells at interphase and mitosis. (A) Workflow of the SILAC experiment. LD and LN, light isotope-labeled cells treated with DMSO and Noc, respectively; HD and HN, heavy isotope-labeled cells treated with DMSO and Noc, respectively. See text for details. (B) The IP products were separated by SDS-PAGE on 10% gels and visualized by Coomassie Blue staining. Arrows indicate the protein bands corresponding to lamins A and C that were excised for MS analysis. (C) Representative mass spectra of selected pairs of non-phosphopeptides and phosphopeptides from lamin A (upper panel) and lamin C (lower panel) in the SILAC experiment. Data were taken from the first experiment, condition 1, except for SGAQASSTPLSPTR of lamin C, which was from the second experiment, condition 1.

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