Table 1 Methylation assay target sequences
From: Transcription start sites and epigenetic analysis of the HSD17B10 proximal promoter
Assay ID | Genomic target sequence | Bisulfite converted target sequence | Pyrosequencing dispensation order |
|---|---|---|---|
ADS2502FS1 | caggcggaatccgccctctggccaaaggactagcgtaccagg | taggyggaattygttttttggttaaaggattagygtattagg | ATCATGTCGTATCGTCTGCTAGACTATGTCGTA |
ADS2502FS1 | ccacgccccacgtctcatgcggcagcggcagacgccccggcccgtcgatccgccccttccgccgcttcgcctcggccaatcaacgagcgcccgcgcccccatcCCCATCCCGTGGAGTGGCCGGCGACAAGATGG (c>g, polymorphism rs1264014) | ttaygtttttaygttttatgyggtagyggtagaygtttyggttygtygtattygtttttttygtygt/gttygtttyggttaattaaygagygagygttygygttttattTTTATTTYGTGGAGTGGTYGGYGATAAGATGG | TCGATCGTCTGATCGTCTATAGTCGTCATGTCGTAGTATCAGTTCGTCAGTCGTCGATCGTTCAGTCGTTCTGTTCGTCATCGATCGATGTCAGTCGTCGTCTATTGATTCGTGAGTAGTCGTCGA |