Figure 5
From: Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana
Identification of the proteolytic activity by overexpressing AtSPP in yeast. (A) Immunoblot analysis of DDM-solubilized membrane fraction overexpressing AtSPP in yeast by using anti-AtSPP. Lane 1, vector control; lane 2, overexpression of AtSPP in yeast; and lane 3, overexpression of HsSPP-GFP in yeast. The filled-arrowhead indicates the monomer and the open-arrowhead indicates the dimer. (B) Evaluation of the proteolytic activity of AtSPP overexpressed in yeast. Proteolytic activity of the membrane fraction of yeast cells overexpressing AtSPP with inhibitor. Lane 1: control (without inhibitor); lane 2: 1 μM(Z-LL)2 small capitor-ketone. The arrow indicates the proteolytic products from myc small capitor-Prl-PP-FLAG peptide detected with the anti-myc antibody.