Figure 2
From: Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana
Construction of GFP-fusion protein expression plasmid. (A) Cloning site of pRS426 GAL1-GFP and overlapping recombination sequence of the primer. (B) SPP genes were amplified with primers described in the Materials and Methods. The Sma I digested vector and PCR products were transformed into Saccharomyces cerevisiae by homologous recombination. yEGFP stands for enhanced green fluorescent protein.