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Figure 1 | BMC Biochemistry

Figure 1

From: Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana

Figure 1

Proteolytic activity of the membrane fraction of “Deep” cell extracts. (A) Immunoblot analysis of the DDM solubilized membrane fraction of “Deep” cells. The location of the AtSPP protein is indicated by the white arrow. (B) Structure of the substrate: the signal sequence of the bovine prolactin mutant (Prl-pp) fused with a myc epitope tag at the N-terminus and a Flag tag epitope at the C-terminus. (C) “Deep” cell membrane fractions solubilized with 0.25% DDM were incubated for 3 h in the presence of the inhibitor. Lane 1: control (without inhibitor); lane 2: 10 μM (Z-LL)2-ketone; and lane 3: 20 μM L-685,458. The arrow indicates the proteolytic products from the myc-Prl-PP-FLAG peptide detected with an anti-myc antibody.

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