Effect of insulin and aspirin on isoproterenol-stimulated glycerol release in rat adipocytes. Cells were incubated for 30 min at 37°C in Krebs-Ringer supplemented with 4% BSA and with the reagents indicated in the Figure. Each bar represents the average ± Standard error (SE) of three to six independent experiments. The letters (a, b, c) represent a significant difference among groups with different treatments by employing one-way Analysis of variance (ANOVA) at p <0.01. Groups with the same letter did not show a significant difference at p <0.05.