Effect of DPI, Cyt c, anti-NOX4 antibody, and AgNO
cAMP-stimulated glycerol release in rat adipocytes incubated in the presence of each of the selected NSAID. Cells were incubated for 30 min at 37°C in Krebs-Ringer supplemented with 4% BSA, 10−3 M Bt2cAMP, and 10−6 M of each NSAID used, as indicated in the Figure. Krebs-Ringer was additionally supplemented with the following: none (control), or 5 × 10−5 M DPI, or 8 × 10−5 M Cyt c, or a 1:6,400 dilution of anti-NOX4 antibody, or 3 × 10−5 M AgNO3. Each bar represents the average ± Standard error (SE) of three to six independent experiments. The letters (a, b, c, d) represent a significant difference among groups with different treatments by employing one-way Analysis of variance (ANOVA) at p <0.01. Groups with the same letter did not show a significant difference at p <0.05.