Concentration-response curve for NSAID on H
generation in rat adipocytes, and in plasma membranes from adipocytes. Panel a, cells were incubated for 10 min at 37°C in Krebs-Ringer-enriched buffer supplemented with 4% BSA plus 10−8 M insulin or NSAID at the concentrations indicated in the figure. Panel b, freshly prepared cell membranes were incubated for 10 min at 37°C in the activation buffer enriched with 3 mM MgCl2 and 4% BSA plus 10−8 M insulin or NSAID at the concentrations indicated in the figure. Insulin-induced H2O2 generation in the absence of NSAID is indicated by an open circle. Assay of H2O2 was conducted as described in experimental procedures and the catalytic reaction was started with 250 μM NADPH. Each point represents the average ± Standard error (SE) of three to five independent experiments. *, p < 0.05 none vs NSAID or insulin.