Figure 4

SKIP and androgen receptor co-immunoprecipitate. Co-immunoprecipitation experiments in COS-7 cells co-transfected with full-length AR WT plasmid and HA-SKIP-plasmid as indicated and described in Materials and Methods. AR-bound and HA-positive (left) or SKIP-bound and AR-positive (N20-antibody) proteins (right) are shown. rIgG stands for rabbit IgG and represents a negative control. Experiments were carried out in presence or absence of 1 nM DHT, as indicated. Lanes 5 and 10 show blotted lysates as input controls for the immunoprecipitation. Approximate molecular weights are as indicated on the left. Arrow shows AR-fragment positive controls, while arrow-head points to HA-SKIP positive controls. Blots are representative of three independent repeats.