Figure 3

SKIP augments AR-dependent transcription in prostate cancer cells. Luciferase reporter transactivation assays were performed in PC3 prostate cancer cells using transiently transfected MMTV- and PSA-luciferase reporters (250 ng/well) with SKIP-pSG5 expression plasmid (200 ng) or empty vector pSG5 (200 ng) as indicated. As PC3 cells have no endogenous AR expression, pCMV-AR WT expression plasmid (25 ng/well) was also transfected in these cells. Data are relative to the no SKIP and no ligand control, which was set as one and expressed as mean ± SEM calculated from 3 independent experiments.