Figure 5
From: The COP1 E3-ligase interacts with FIP200, a key regulator of mammalian autophagy
Effect of COP1 on UV-mediated tumorigenesis. NIH3T3 cells were transfected with expression vectors encoding HA-GFP and HA-GFP-fused COP1 (wild type and SA mutant) and selected. After exposure to UV (50 J/m2) and recovery culture, the cells (ca 106) were subcutaneously injected into NOD-SCID mice. At 2.5 months post-injection, mice were sacrificed and the weights of the tumors were measured. Note that, except for the cells expressing the ectopic wild-type COP1 with UV treatment, no trace of the cells injected was detected. Therefore, the sizes and weights of the tumors in those cases are actually 0. Cells (ca 106) recovered from the tumors were injected into NOD-SCID mice, and the size of the tumor was measured after 3 weeks (right column). The data are averages for three independent experiments. Photos of the tumors are also shown (upper).