Figure 1
From: The COP1 E3-ligase interacts with FIP200, a key regulator of mammalian autophagy
FIP200 interacts with COP1. (A) Y190 yeast cells were transfected with pAS2 vectors containing full-length and deletion mutants (ΔWD40 and ΔRING) of COP1 together with pACT vectors containing FIP200 and JunD. Transfectants were grown on a Trp- Leu- His- selection medium and tested for β-galactosidase activity (top). Schematic structure of COP1 mutants (bottom). (B) GST and GST-FIP200 recombinant proteins were immobilized on glutathione beads (bottom panel, separated by SDS-PAGE and stained with CBB) and mixed with cell lysate containing ectopically expressed HA-tagged wild-type and ΔWD40-mutant form of COP1. Bound proteins were analyzed by Western blotting using antibody against COP1 (upper panel).