Growth and development of deletion mutants of Ddpngase . (A) Comparison of growth profiles of wild type ([act15/eyfp]/Ax2), knockout (png-/Ax2), overexpresser [act15/png-eyfp]/Ax2 and random integrant (RI) strains. Generation time (doubling time) was calculated in logarithmic phase cultures2.5-4x106 cells/ml) using the formula: generation time = (t2-t1) x [log2/log (q2/q1)]. (B) Comparison of developments of wild type (WT), knockout (KO), and overexpressing (OE) strains are showed. Few developmental stages like mound stage (i, ii, iii); migrating slug stage (iv, v, vi) and fruiting body stage (vii, viii, ix) phenotypes are shown. Scale bar 1000 μm.