Tertiary structure prediction of putative Dd PNGase. (A) Multiple alignment of of the conserved core domain of D. discoideum and M. musculus is shown. The red coloured boxes show the conserved catalytic triad residues corresponding to C210, H237, and D252 from D. discoideum. The two CXXC motifs are shown in blue boxes. The conserved tryptophan (W239, W248), arginine (R229) and glutamate (E241) are shown in black boxes. The numbers in the left represent the position of the amino acid in the respective full length protein sequences. (B) Tertiary structure homology prediction model of Dd PNGase (core domain) based on mouse PNGase (2f4m-A) as a template was created by SWISS MODEL and viewed using MOLMOL.