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Table 1 List of primers used for mutagenesis

From: Phosphatidylcholine formation by LPCAT1 is regulated by Ca2+ and the redox status of the cell

Purpose

Primer sequence (5′ to 3′)

saturated mutagenesis of

GCCTCAGATAATGATTTTTCCAGAAGGAACTNNKACAAA-

Cys-211 codon

TAGGACCTGCCTCA

Cys-211 to Ser

GATTTTTCCAGAAGGAACTAGTACAAATAGGACCTGCCT

Glu-403 to Ala

TGAGATTGACCTTCGTGCATATGTGGTCGCCTTGT

Cys-216 to Ala

GAACTGCTACAAATAGGACCGCCCTCATTACCTTCAAACCTG

Cys-263 to Ala

AATCCTGTGGCTCACTCTGGCCCAGTTTCAAAACCAAGTG

Cys-278 to Ala

GTGGAAATTGAATTTCTGCCTGTGTATGCCCCTTCTGAAGAGG

Cys-314 to Ala

ACTATACATTTGAGGACGCCCAGCTGGCTCTGGCAG

Cys-330 to Ala

CTTGCCTGCTGACACCGCCCTGCTAGAGTTTGCC

Cys-401 to Ala

GCCTTGTCTGTGGTGGCCAGGCCATCCCAGAC

Cys-443 to Ala

GAGGCCAACCTGTCCGCCATCCTCAAGACTGC

Cys-477 to Ala

AATCACCTTTGATGACTTCGCCGGGTTTGCGGAAATGTAC

Cys-501 to Ala

GACACATTTCGACAGCGCTGCACAGACACCCCCA

Cys-514 to Ala

CAACTCCCAATGGCTTCGCCATTGACTTCAGCCCTG

Cys-530 to Ala

ACTTTGGGAGAAAGAATTCTGCTAAGAAAGCGGACTAGCCTC