Figure 6

Activity of Cysteine substitution mutant enzymes. Alanine scanning of the 11 cysteines present in the active form C²¹¹T was performed to generate a collection of C-to-A mutant forms. The list of clones and their mutations are presented in Additional file 2: Table S1. (A) Clones were tested for production of the mutant protein (top gel) and for their lysoPC-acyltransferase activity (bottom) as described in the legend of Figure 4A. The row number (4 to 17) indicates the clone listed (row 4 to 17) in Additional file 2: Table S1. (B) Activity rate measurements were performed as described in the legend of Figure 4B. In addition to LPCAT1 and C²¹¹T, rate values for the mutant enzymes with significant acyltransferase activity as shown on panel A, [C²¹¹T C⁴⁴³A, row 4], [C²¹¹T C^(314,457)A, row 5], [C²¹¹T C(^216,443,514)A, row 6] and [C²¹¹T C^{(216,314,443,501,514)}A, row 7], were determined. Protein expression and activity of the form C²¹¹T, C⁵⁰¹A are presented in Additional file 1: Figure S1. Values are reported relative to the activity rate obtained with LPCAT1 enzyme. The standard deviation of at least 3 different measurements is indicated as error bars.