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Figure 1 | BMC Biochemistry

Figure 1

From: The ORF2 glycoprotein of hepatitis E virus inhibits cellular NF-κB activity by blocking ubiquitination mediated proteasomal degradation of IκBα in human hepatoma cells

Figure 1

Heterologous expression of ORF2 protein inhibits NF-κB activity. (A) Huh7 cells were cotransfected in duplicate with NF-κB CAT reporter and pSGI vector or pSGI-ORF2 expression plasmid. 48 hour post transfection, cells were treated with 100 ng/ml TPA for 30 minutes and CAT activity was assayed. Spot intensity was quantified using NIH Image program and percentage CAT activity was calculated assuming the highest value as 100%. Graph represents mean ± SEM of 3 independent sets of experiments. (B) Huh7 cells were cotransfected in duplicate with NF-κB CAT reporter, IKKβ expression plasmid and pSGI vector or pSGI ORF2 expression plasmid. 48 hour post transfection, CAT activity was assayed. Graph represents mean ± SEM of 3 independent sets of experiments.

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BMC Biochemistry

ISSN: 1471-2091

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