Figure 1

Phylogenetic analysis of putative avian wax synthases in comparison to different acyltransferases from human, plants, yeast, Tetrahymena and bacteria. The phylogram shows the relation of the analyzed avian proteins with acyltransferases from different organisms. The tree is based on the alignment of the following sequences (NCBI accession numbers are given): HsAWAT1 [Homo sapiens, NP_001013597.1], HsAWAT2 [H. sapiens, NP_001002254.1], HsMOGAT3 [H. sapiens, NP_835470], HsMOGAT2 [H. sapiens, NP_079374], HsMOGAT1 [H. sapiens, NP_477513.2], HsACAT1 [H. sapiens, NP_003092.4], HsACAT2 [H. sapiens, NP_003569.1], HsDGAT2 [H. sapiens, NP_115953.2], HsDGAT1 [H. sapiens, NP_036211.2], SchWS [Simmondsia chinensis, AF149919_1], EgWS [Euglena gracilis, ADI60058.1], PxhWS [Petunia x hybrida, AAZ08051.1], AcWS/DGAT [Acinetobacter sp. ADP1, YP_045555.1], GgWS1 [Gallus gallus, XP_424082.2], GgWS2 [G. gallus, JQ031643], GgWS4 [G. gallus, XP_419207.1], GgWS5 [G. gallus, NP_001026192.1], AdWS5 [Anser domesticus, JQ031647], TaWS5 [Tyto alba, JQ031646], AdWS4 [A. domesticus, JQ031643], TaWS4 [T. alba, JQ031645], HsTMEM68 [H. sapiens, Q96MH6.2], GgDGAT1 [G. gallus, JQ031642], Tetrahymena WS [Tetrahymena thermophila, XP_001027910, XP_001026090, XP_001008104, XP_001019739], MhWS1 [Marinobacter hydrocarbonoclasticus, ABO21021.1], Arabidopsis WSD [Arabidopsis thaliana, NP_568547.1, NP_177356.1, NP_850307.1, NP_200151.2] MrDGAT2B [Umbelopsis ramanniana, AAK84180.1], MrDGAT2A [U. ramanniana,AAK84179.1], ScDGA1p [Saccharomyces cerevisiae, NP_014888.1], ScARE1p [S. cerevisiae, CAA42296.1], ScARE2p [S. cerevisiae, CAA96298.1], Arabidopsis WS [A. thaliana, NP_200345.1, XP_002866091.1, NP_200349.1, NP_200346.1]. The scale corresponds to amino acid substitutions per site in the alignment of 41 sequences with a total of 188 positions. Numbers at the branches are bootstrap values indicating the probability of this relationship in %. Values above 95 can be regarded as correct. The dendrogram was created with ClustalX2 and MEGA5 software.